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Antimicrobial susceptibility profiles and carbapenemase detection in Klebsiella pneumoniae strains. (A) Minimum inhibitory concentrations (MICs, μg/ml) for a panel of antibiotics were determined for CRKP ST101, CRKP ST307, and the reference strain K . pneumoniae ATCC 13883 using broth microdilution or automated systems, as appropriate. (B) Detection of carbapenemase enzymes was performed using a lateral flow <t>immunoassay.</t> Test bands indicate the presence of specific carbapenemases: IMP (I), VIM (V), NDM (N), KPC (K), and OXA-48-like enzymes (O). The control (C) band serves as an internal validity marker for test performance.
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Antimicrobial susceptibility profiles and carbapenemase detection in Klebsiella pneumoniae strains. (A) Minimum inhibitory concentrations (MICs, μg/ml) for a panel of antibiotics were determined for CRKP ST101, CRKP ST307, and the reference strain K . pneumoniae ATCC 13883 using broth microdilution or automated systems, as appropriate. (B) Detection of carbapenemase enzymes was performed using a lateral flow immunoassay. Test bands indicate the presence of specific carbapenemases: IMP (I), VIM (V), NDM (N), KPC (K), and OXA-48-like enzymes (O). The control (C) band serves as an internal validity marker for test performance.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Meropenem/vaborbactam activity against carbapenem-resistant Klebsiella pneumoniae from catheter-related bloodstream infections

doi: 10.3389/fcimb.2025.1616353

Figure Lengend Snippet: Antimicrobial susceptibility profiles and carbapenemase detection in Klebsiella pneumoniae strains. (A) Minimum inhibitory concentrations (MICs, μg/ml) for a panel of antibiotics were determined for CRKP ST101, CRKP ST307, and the reference strain K . pneumoniae ATCC 13883 using broth microdilution or automated systems, as appropriate. (B) Detection of carbapenemase enzymes was performed using a lateral flow immunoassay. Test bands indicate the presence of specific carbapenemases: IMP (I), VIM (V), NDM (N), KPC (K), and OXA-48-like enzymes (O). The control (C) band serves as an internal validity marker for test performance.

Article Snippet: Lateral flow immunoassay was used to detect clinically relevant carbapenemases, specifically KPC, IMP, NDM, VIM, and OXA-48 (Coris BioConcept, Belgium).

Techniques: Control, Marker